Hi,
I have a mutant mouse model in which there are increased auto-antibodies against insulin in the blood. Does someone know why these autoantibodies could be elevated in mouse blood?
Thank you.
Hi, I have performed a glucose tolerance test (GTT) on mutant vs WT mice and I have no statistical difference in glucose levels (mg/dL) during the test between the mutant and WT mice, and no...
10 November 2017 5,686 3 View
Hi, From the litterature I found that Glucose Tolerance Test is often performed on mice fasted 6h, while the fasting period for a Pyruvate Tolerance Test is overnight in most of the articles. Do...
10 November 2017 5,892 2 View
Hi, I performed several glucose tolerance tests (GTT) to assess glucose tolerance in mutant mice compared to WT mice. Each test was done on 5-6 mutant mice and 5-6 WT mice at the same time. All...
10 November 2017 795 4 View
Hi, I have a decrease of 1/3 to 1/2 in the gene expression of gluconeogenesis enzymes (Glucose-6-phosphatase, Fructose-1,6-bisphosphatase, Phosphoenolpyruvate carboxykinase and Pyruvate...
06 July 2017 5,980 6 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...
02 March 2021 6,865 1 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View