Hi,
I have a mutant mouse model in which there are increased auto-antibodies against insulin in the blood. Does someone know why these autoantibodies could be elevated in mouse blood?
Thank you.
Hi, I have performed a glucose tolerance test (GTT) on mutant vs WT mice and I have no statistical difference in glucose levels (mg/dL) during the test between the mutant and WT mice, and no...
10 November 2017 5,728 3 View
Hi, From the litterature I found that Glucose Tolerance Test is often performed on mice fasted 6h, while the fasting period for a Pyruvate Tolerance Test is overnight in most of the articles. Do...
10 November 2017 5,939 2 View
Hi, I performed several glucose tolerance tests (GTT) to assess glucose tolerance in mutant mice compared to WT mice. Each test was done on 5-6 mutant mice and 5-6 WT mice at the same time. All...
10 November 2017 888 4 View
Hi, I have a decrease of 1/3 to 1/2 in the gene expression of gluconeogenesis enzymes (Glucose-6-phosphatase, Fructose-1,6-bisphosphatase, Phosphoenolpyruvate carboxykinase and Pyruvate...
06 July 2017 6,027 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
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05 August 2024 4,407 0 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View