Hi,
I have a decrease of 1/3 to 1/2 in the gene expression of gluconeogenesis enzymes (Glucose-6-phosphatase, Fructose-1,6-bisphosphatase, Phosphoenolpyruvate carboxykinase and Pyruvate carboxylase) in mice liver between my test condition and my control condition and I would like to know if it can lead to a reduction of the flux of the pathway or not? Also is one of these enzymes more critical than the others in the pathway?
Thank you.
To further elucidate the possible metabolic outcome associated with changes in the gluconeogenesis pathway, protein expression of these enzymes is warranted. As for the second question, Fructose-1,6-bisphosphatase (FBP1) is the rate-limiting enzyme in gluconeogenesis.
I agree with Rola, however you could make the claim that these data suggest that gluconeogenesis is impaired in the in the test subjects. Protein quantification will likely mirror these results and strengthen your argument.
Hi there,
What about protein level? Transcription level is not always correlated to translation level. Furthermore enzyme activity might also be regulated at the protein level. So it's difficult to conclude anything from your data.
Hi,
Thank you for your comment. I still have to do the protein quantification.
In addition to what my colleagues have stated, One may actually claimed inhibition of gluconeogenesis however protein quantitation would brings out the beauty of the work and a better conclusion. Thanks
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