How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Does anyone here uses the Software for Roche LightCycler 2.0 instrument?

Hi everyone, If anyone here uses Roche LightCycler 2.0 instrument for qPCR experiments, I would appreciate if they message me. I have some questions about it. Best - Navid

12 October 2020 6,234 2 View

What is an acceptable FDR% for proteomics data publication?

Is there an established convention on this? I have heard from experts that 1% FDR is recommended. I was wondering can we report protein IDs at 5% FDR. Is there any published guidelines on this? If...

25 August 2020 1,231 4 View

Does the expiration date affect the binding capacity of Ni-NTA?

Hello! I have some expired Ni-NTA resin (QIAGEN), and I need to purify my His-tagged protein. Does the expiration date affect the binding capacity of Ni-NTA? Thank you in advance

11 June 2020 5,773 5 View

How can concrete be modeled with a GFI in ABAQUS?

Hello dears I am modelling concrete in the Abaqus and need to know GFI behavior and parameters them. How can concrete be modeled to the ABAQUS? Thanks

03 March 2020 4,116 3 View

Is there any good free software to simulate electron trajectory in non-uniform electric field?

Need to simulate electron trajectory in non-uniform electric field.

08 February 2020 8,963 3 View

New project in Cathodic Protection?

What fields (topics) do you suggest to work as a metallurgy and Technical Inspection master degree Student in Cathodic Protection? Thank you kindly

17 January 2020 4,201 5 View

Does anyone have a good protocol for dissecting the second order mouse pulmonary artery?

Does anyone have a good protocol for dissecting the second order branch of the pulmonary artery from mouse. I am interested in performing some pressure myography studies.

18 December 2019 9,030 1 View

Should the effective stresses be constant during the effective stress numerical analysis?

Assuming running the undrained effective stress analysis in FLAC3D via Config FLUID using the effective stiffness and strength parameters, are we expecting the constant effective stresses in the...

15 July 2019 6,096 3 View

How to model the distribution of the population from a facility to other near facilities (for example a school) when it is decided to be destroyed ?

how can we model the distribution of the population from a facility to other near facilities (for example a school or a hospital) when it is decided to be destroyed

25 June 2019 4,220 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View