Can anyone help me with Sciex Q-TOF? What are Gas 1 and Gas 2, and also what is curtain gas?

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What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

How can I find my published papers' total read, citation or any other scores?

21 July 2024 9,802 3 View

I need the Knowledge, Attitudes, and Practices (KAP) Questionnaire for my thesis project. Can anyone send it to me?

I indeed looked for it everywhere but in vain. Many thanks in advance.

25 April 2024 7,140 2 View

Hi, plaese guide anyone, how can we find the work function from XPS valance band potential?

Answer need on urgent base please.

16 January 2024 5,349 5 View

IAST selectivity and heat of adsorption?

Dear all, I have worked on the gas uptake with MOFs and now I need to calculate IAST selectivity and HOA. I've used python code which is published in some papers but it did not work for me. I...

25 December 2023 5,090 0 View

How to fix cryopreserved cells?

We have some frozen cell-lines in freezing medium (10%DMSO in FBS), which we want to fix using 10% NBF and eventually make FFPE blocks with. Would it be ok to just thaw them rapidly and wash them...

22 November 2023 5,110 1 View

How can I disrupt extracellular vesicle (EV) membrane in order to measure their protein content?

I have read that I can use RIPA buffer for EV membrane disruption. Does this have to be followed by ultra centrifugation? Does anyone have a protocol for this? Thanks.

26 July 2023 2,465 5 View

Which kind of body fluid is applicable to measure MicroRNAs ? , AND What are challenges to detect MicroRNAs in body fluid samples?

To measure and detect MicroRNAs, researchers face many challenges, Some of them are: 1- Small size 2- Similarity in sequence in the same family 3- A tiny amount in the sample

19 June 2023 6,033 3 View

What to do for smooth peeling off of semi refined carrageen film from plastic petridish?

I have prepared thin films from semi refined carrageenan with hydrophilic cross linkers. But smooth peel off has been the issue for a long time.

06 June 2023 6,013 2 View

Fatigue and determination of life in the metal support structure of pressure vessels?

I am conducting an earthquake-induced fatigue study on the metal supports of the pressure vessels, which is responsible for the stability of the structure.

11 May 2023 4,648 2 View

AGILENT HPLC 1100 SERIES , any expert available please?

AGILENT HPLC 1100 SERIES operation assistance needed.

25 July 2024 10,045 2 View

What are these clumps in my transfected PBMCs?

Hello everyone. I cultured isolated PBMCs in Erythroid Expansion Medium for two weeks and then performed electroporation in my expanded PBMCs in order to reprogram them. The first 24h after the...

22 June 2024 346 4 View

Need to obtain concentration of DNA/RNA?

I need to use a photometer, so i have Cary Agilent and i would like to know what kind of cuvettes and its dimensions and volume i have to order. It's about RNA and DNA samples. Which are you...

11 June 2024 711 1 View

How to choose the time and acid-base environment for the Clayson Schmidt reaction?

I want to replicate the third step, but there seems to be no reaction in an alkaline environment. However, sodium hydroxide is used in the literature. Should I switch to an acidic environment

05 June 2024 9,358 1 View

What does this peak in our Bioanalzer profile from Agilent 2100 mean? Why does single peak show up where 28srRNA is expected? Why don't we see 18s?

Hello fellow researchers, Why don't we see two rRNA peaks as is standard? Our sample was a total RNA prep from A549 cell line. We ran our samples on a pico chip for 2100 Agilent bioanalyzer...

28 May 2024 7,665 0 View

Inductive or Deductive?

Using thematic analysis on our research on Identity Negotiation of Female Gaming Streamers in our local area, we have developed INT elements (communication process, biculturalism, and...

18 May 2024 9,134 2 View

What are the key and important parameters in the design of switches based on photonic crystal?

What are the key and important parameters in the design of switches based on photonic crystal? How can we have an ideal switch?

09 May 2024 7,074 2 View

The modal switching problem for multimodal robots ?

How does an amphibious robot with two sets of power units rationally switch between them? Or the modal switching problem for multimodal robots?

05 May 2024 4,624 2 View

What is the best LC/MS system for surfactant analysis?

We are an R&D laboratory focused on anionic, cationic, and zwitterionic surfactants and usually tackle surfactant synthesis and analysis of products from customers. In particular, we deal with...

29 April 2024 8,925 1 View

Substitutes for Dako anti-HBcAg rabbit polyclonal?

I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...

28 April 2024 3,860 1 View