In Order to undergo qRT-PCR, after RNA extraction and cDNA synthesis, if I would like to collect the samples to be conducted through the same run.
Hi
I think you can get the cDNA at -20 hold it for long. Even at -80 RNA remains too long.cDNA is in fact a double-stranded DNA to RNA is not sensitive.cDNA synthesis few months ago I got the result I'm keeping in -20 temperatures.
Hope Luck
Hellow,
Both DNA and cDNA are high stable molecules. I think they can be store for a very long time at -20ºC.
Hi,
cDNA can be stored for years in -20°C. But for proper qPCR results I usually try not to store it longer than several weeks. But if you work properly during cDNA synthesis using nuclease free tubes, tips and buffers there is no problem in storing it for a longer time.
RNA always should be stored in -80°C, for storage of genomic DNA I usually choose 4°C to avoid induction of breaks in the DNA.
Good luck with your experiment!
You can store cDNA at -20 or -80 for longer time but try to avoid repeated freezing and thawing cycles. Ideally, should be stored in aliquots.
- Badges
- Science method