Mohamed Nour Eldin

7 Questions 10 Answers 0 Followers

Questions related from Mohamed Nour Eldin

How can I store cells for using them for ELISA later?

After a cell culture experiment, how can I store my cells to undergo ELISA later on? Is it better to store the cells in 0.9% NaCl saline or to store them as a pellet. Also at -80 C or -20 C?

01 January 2015 2,074 4 View

If I would like to make ELISA for cell culture to estimate caspase 3, should I start with a certain count of cells?

Does this count differ from cell line to another or from parameter to another?

12 December 2014 6,019 5 View

What is the recommended conc. of Trichostatin A (TSA) to be added to the cell culture?

In order to treat a cell culture with TSA for re-expression of some genes without direct cytotoxic effect.

12 December 2014 398 2 View

For how long can I store cDNA at -80C?

In Order to undergo qRT-PCR, after RNA extraction and cDNA synthesis, if I would like to collect the samples to be conducted through the same run.

12 December 2014 8,853 10 View

What is the stability of 2-deoxy 5-Azacytidine in DMEM and DMSO ?

It is expected to store the prepared solution at -20C for 2 months.

09 September 2014 1,326 2 View

Can anyone suggest biochemistry journals with no publication charge?

In order to publish my new research , I need biochemistry or molecular biology journals with no publication charge

06 June 2014 632 8 View

How can I find a selective primer for Estrogen receptor beta 1 (not other splice variants) for quantitative real time PCR?

For determining the amount of mRNA as indication for gene expression.

01 January 2014 9,836 0 View