Zahra Hassani

5 Questions 9 Answers 0 Followers

Questions related from Zahra Hassani

Why can't I extract plasmids from bacteria DH5 alpha?

Hi I pTZ57R/T Fermentas vector to clone genes in the bacterium E. coli was used.Now I can not do of a single colony obtained plasmid extraction. I have 16 hours in a single colony was grown labial...

04 April 2015 4,709 6 View

Why can I not find any colony which contains the gene?

I have a 1300bp gene using vector psk my cologne. But after steps gene transfer colony can find her?After culturing the transfection bacteria in a medium containing IPTG and Xgal and...

02 February 2015 2,221 3 View

How to combat Beauveria bassiana fungus infection in my tobacco culture?

Hi All my tissue culture infected with the fungus.(Culture tobacco)I also used to combat fungal antibiotic Dodin but I had no effect

01 January 2015 1,767 10 View

What can I do about the concentration of DNA extraction bacteria without the use of NanoDrop and spectrophotometry determination?

How can I do agarose gel electrophoresis, and then compare the bands to the gene ladder and detect the concentration of the gene?

12 December 2014 8,647 10 View

Why can not ligation and transfer gene transfer in bacterial colonies can never take?

I do Cloning: in ligation: 15 µ DNA 5 µ Plasmid (pks), 2/5 µ H2O, 0/5 T4, Buffer 2µ Added and 90 Minute at a temperature of 16 ° C gave. The resulting product is then transferred into competent...

12 December 2014 8,227 6 View