Gertrud Wiedemann

5 Questions 23 Answers 0 Followers

Questions related from Gertrud Wiedemann

DNA extraction from human hair using QIAamp DNA Mini Kit?

Does anone have experience using the QIAamp DNA Mini Kit for the extraction of DNA from human hair? How much of starting material will be necessary. How is the pretreatment of the sample done...

10 October 2018 4,688 1 View

Any experience with IDH1/IDH2 ddPCR assays by Biorad?

Dear all, Does anyone use the ddPCR assays for detection of IDH1 p.R132C, IDH1 p.R132H, IDH2 p.R140V? I would appreciate any feedback by people using these tests for mutation detection and follow...

07 July 2018 1,524 0 View

Is it possible to sequence products for fragment analysis?

Dear all, is it possible to do Sanger sequencing from samples generated for fragment analysis thus being labelled with a fluophore? If it is possible, do you have any experience if...

05 May 2018 6,110 5 View

Experiences using Oneservice Blood Mini QC kit compared to QIAamp DNA Blood Mini Kit on Qiacube?

Dear all, has anyone used the Blood Mini QC kit from Oneservices with a Qiacube? If yes, how does this kit perform compared to the Qiagen QIAamp DNA Blood Mini Kit regarding yield and sample...

03 March 2018 7,182 1 View

RT with Taqman Reagents: conditions for random hexamer primers 48°C vs 37°C?

Dear all, does anyone have experience how the cycling conditions influence cDNA synthesis using the Taqman Reverse Transcription Reagents with random hexamers for qRT-PCR applications. For years I...

07 July 2016 5,415 1 View