We are detecting different samples, tissues and cells, to detect inflammatory cytokines. We are getting good results for B-actin, NF-KB and IL-12 but after trying so many times we were unable to detect IL-1 B and IL-6 using primary antibody IL-6 from ABCAM and IL-1B from Thermo and Santa, and detecting by the dry method of Western Blot. Is there any special treatment to detect these antibodies?

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