Jose Sofia Ruffino

3 Questions 24 Answers 0 Followers

Questions related from Jose Sofia Ruffino

RNA extraction from muscle - low 260/230 ratio - any thoughts?

I have extracted RNA from human muscle biopsy samples (~10-20mg starting material) using TRIzol and the chloroform/phenol method of extraction for use in RT-qPCR (SYBR green method). The samples...

15 July 2014 2,042 5 View

Which molecular weight of glycogen should be used for glycogen samples from the abcam glycogen kit?

I am measuring glycogen in freeze dried human vastus lateralis muscle samples using the abcam glycogen kit. This kit provides glycogen concentrations in ug/ul but publications report glycogen...

02 April 2014 3,352 5 View

Protein lysis and storage for Western Blot: Is boiling necessary prior to storage?

I am currently in the process of propagating primary human macrophages for protein samples to be used in Western Blot. With cultured cells I have used the RIPA buffer method (RIPA buffer plus...

18 January 2013 599 25 View