Pedro Escoll

2 Questions 11 Answers 0 Followers

Questions related from Pedro Escoll

Must the infection of human monocyte-derived macrophages always be done under serum-free conditions?

I'm infecting human macrophages with an intracellular bacteria and I have performed the infection always under serum-free conditions. I differentiate the macrophages from blood CD14+ monocytes...

13 February 2013 6,815 9 View

How to decontaminate supernatants of infected cells in order to measure cytokines by Cytometric Bead Array?

I want to measure cytokine production of infected macrophages using the human CBA inflammation kit (BD), but the cytometer that I want to use is in a P1 room, and I'm not allowed to acquire the...

23 November 2012 3,961 11 View