Paul Dias

4 Questions 74 Answers 0 Followers

Questions related from Paul Dias

Are there any easy method to detect oxygen levels in RBC (invitro)?

I wanted to measure oxygen caring capacity of RBCs invitro. Could anyone help me with protocol if available with you

25 January 2016 4,744 13 View

Determination of eNOS dimer-monomer levels in endothelial cells - can anyone help?

I'm attempting to check dimer-monomer ratio of eNOS protein using LT-PAGE approach. Could anyone share the protocol. Most of the papers I searched have done monomerization by addition of...

25 September 2013 228 11 View

Can anyone help me with a TRAP (telomere repeat amplification protocol) assay?

I am using millipore kit (TRAPeze Kit) to determine telomerase activity. I am getting non-specific multiple bands in the negative control (only CHAPS lysis buffer). Is this common? Are there any...

07 August 2013 3,035 8 View

Could anyone advise on determination of Calcium flux using Fluo-4AM dye in endothelail cells?

Could anyone help me in determination of Calcium flux using Fluo-4AM dye in endothelail cells. It would be great if i can get the protocol

06 August 2013 7,679 0 View