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Questions related from James John
Usually i had extracted DNA from soil sample using power soil Qigen kit and got upto 700 ng/ul of DNA. I do have a soil sample which has pH of 2.3 higlhiy acidic and one with 12.3 which is...
18 March 2021 6,166 14 View
I have a target of 176 bp which got a Ct value between 24-25 (three replicates), i also ran gel from PCR amplification and from amplicon of qPCR after melting curve melting curve has a sinlge...
27 January 2021 4,035 2 View
Hi every one Can anyone help me to prepare a Mutation in A. baumannii ATCC 17978 using transposon? I want to find out those gene involved in resistant to aminoglycosides Regards
04 December 2019 9,424 0 View
I checked the apoptotic effect of a natural molecule on cell line. In my western result I do get over expressed PARP Clvd and caspase 3 positive at 24 hours after treatment. When i checked level...
03 August 2016 1,282 3 View
I have successfully made a construct for knock-out by overlapping extension PCR with Kanamycin selection marker (RR-Kan-RF, FF=312bp, RF=313bp, Kan=937bp) for Acinetobacter baumannii (Gram -ve). I...
30 September 2015 8,691 1 View
I am searching for a reference article which uses LC-MS/MS analysis to make Quantification with label free approach using Progenesis QI software. I have two set of protein profile with control...
30 June 2015 5,690 3 View
I am working to extract the whole protein from staphylococcus aureus. the steps include treating cell pallets with lysostaphine followed by water bath sonication. The collected supernatant is...
24 April 2015 2,781 4 View
Can anybody provide me with the best protocol to be used for protein extraction form staph aureus?
27 February 2015 5,795 3 View
I am finding it difficult to dissolve DAPI in water as many protocol mention distilled water or PBS How to dissolve DAPI stain?
25 February 2015 611 5 View
I do have ECL chemiluminescence for blotting. Finding difficult with x ray film developer. Would it be possible to use Gel doc (Used for electrophoresis gel imaging) for blot imaging?
19 February 2015 8,895 1 View
I would like to know any simple stain or test bu which i could stain or quantify only the EPS or biofilm present and not bacteria present in it.
31 January 2015 5,007 9 View
How long can I wait to develop an x ray film after my blotting paper is treated with ECL pierce (Chemiluminescence).
22 January 2015 2,136 3 View
I have a lead molecule which has an inhibitory effect on Staph and I would like to know the proteins which are interacting with the lead molecule What can I perform to find out which pathways...
04 November 2014 5,998 6 View
I am treating bacteria with my molecule and want to see proteins which are suppress or depress by the molecule in staph auresu bacteria. I would like to know what all techniques could be used for...
22 August 2014 6,588 3 View
I have the custom primary and secondary antibodies-HRP tagged got from market, unquoted ELISA plates and target sample also. Now if want to develop in house ELISA what additional thing do I need...
15 July 2014 1,904 4 View
I need to extract membrane and cytoplasmic protein from gram positive bacteria for 2D gel.
12 February 2014 712 1 View
Dear colleagues, I am currently working on a project focused on quorum sensing and virulence gene regulation in Pseudomonas aeruginosa and related bacteria. I am seeking the following reporter...
01 January 1970 6,967 2 View
