James John

13 Questions 24 Answers 0 Followers

Questions related from James John

How to extract DNA from a soil with acidic pH?

Usually i had extracted DNA from soil sample using power soil Qigen kit and got upto 700 ng/ul of DNA. I do have a soil sample which has pH of 2.3 higlhiy acidic and one with 12.3 which is...

18 March 2021 6,148 14 View

Low Ct value in qPCR but no band in gel?

I have a target of 176 bp which got a Ct value between 24-25 (three replicates), i also ran gel from PCR amplification and from amplicon of qPCR after melting curve melting curve has a sinlge...

27 January 2021 4,017 2 View

Mutation using transposon?

Hi every one Can anyone help me to prepare a Mutation in A. baumannii ATCC 17978 using transposon? I want to find out those gene involved in resistant to aminoglycosides Regards

04 December 2019 9,409 0 View

In what condition Caspase 3Cleaved would be positive even before Caspase 8?

I checked the apoptotic effect of a natural molecule on cell line. In my western result I do get over expressed PARP Clvd and caspase 3 positive at 24 hours after treatment. When i checked level...

03 August 2016 1,267 3 View

How do I analyze LC-MS/MS data for a with label free approach using progenesis QI software?

I am searching for a reference article which uses LC-MS/MS analysis to make Quantification with label free approach using Progenesis QI software. I have two set of protein profile with control...

30 June 2015 5,676 3 View

Can any body suggest how to solubilize bacterial protein extracted by acetone precipitation?

I am working to extract the whole protein from staphylococcus aureus. the steps include treating cell pallets with lysostaphine followed by water bath sonication. The collected supernatant is...

24 April 2015 2,762 4 View

Can anybody provide a protocol for protein extraction of staph aureus bacteria?

Can anybody provide me with the best protocol to be used for protein extraction form staph aureus?

27 February 2015 5,773 3 View

How to dissolve DAPI stain?

I am finding it difficult to dissolve DAPI in water as many protocol mention distilled water or PBS How to dissolve DAPI stain?

25 February 2015 600 5 View

Can anybody tell if there is any stain which can stain only EPS and not bacteria present in biofilm?

I would like to know any simple stain or test bu which i could stain or quantify only the EPS or biofilm present and not bacteria present in it.

31 January 2015 4,991 9 View

Can I store blotting paper after treating with ECL pierce, in case I want to develop it later on with X ray film?

How long can I wait to develop an x ray film after my blotting paper is treated with ECL pierce (Chemiluminescence).

22 January 2015 2,123 3 View

How can I know the target protein for my molecule in staph auresu?

I am treating bacteria with my molecule and want to see proteins which are suppress or depress by the molecule in staph auresu bacteria. I would like to know what all techniques could be used for...

22 August 2014 6,571 3 View

How does one develop in house ELISA?

I have the custom primary and secondary antibodies-HRP tagged got from market, unquoted ELISA plates and target sample also. Now if want to develop in house ELISA what additional thing do I need...

15 July 2014 1,893 4 View

Can anybody provide a highly efficient manual method to extract bacterial protein for 2D gel electrophoresis?

I need to extract membrane and cytoplasmic protein from gram positive bacteria for 2D gel.

12 February 2014 697 1 View