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Questions related from Noman Khan
I read some papers they observe anticancer dugs effects using through western blot assay. They observe PARP or caspases or BAX or BCL-2 (apoptosis related protein) after long time point 24 or 48...
22 February 2015 8,217 4 View
I want to check the proliferative effects of some anticancer drugs (more than 50). I observe through CCK-8 or MTT assay. What is the best assay to check their proliferative properties other than MTT.
22 February 2015 2,004 13 View
I got this cell line from 2 different sources. It primarily belongs to ATCC and culture according to ATCC guideline. But in both cases cells die after 2nd passage. I am using 10% FBS in RPMI media.
07 October 2014 7,241 3 View
I read some papers in which they used sigma C2139 (which is collagenase VIII) while some papers reported colagenase III. Can I use any collagenase to isolate interstitial cells for flow cytometry...
07 April 2014 9,293 2 View
We are detecting different samples, tissues and cells, to detect inflammatory cytokines. We are getting good results for B-actin, NF-KB and IL-12 but after trying so many times we were unable to...
03 April 2013 8,824 16 View
I previously worked on the human lung cancer cell line A549 in BALB/c nude mice. Now I want to find out about the inflammatory response of some chemotherapeutic agent in BALB/c mouse by using a...
14 January 2013 9,439 2 View