Noman Khan

7 Questions 6 Answers 0 Followers

Questions related from Noman Khan

Is it appropriate to use only GR-1 or F4/80 FITC labeled antibody( without cd45 or cd11b) to isolate mice lung neutrophils or macrophages?

to find out role of Neutrophils and machrophages in lung inflammation after drug treatment isolated mice lung single cells and by the help of Percoll separate leukocytes..than stained with GR-1...

01 April 2015 8,992 3 View

Why do apoptotic proteins like PARP and caspases observe after 24 or 48 hrs while MAPK/PI3K pathways proteins observe after 1 or 2 hrs in vitro?

I read some papers they observe anticancer dugs effects using through western blot assay. They observe PARP or caspases or BAX or BCL-2 (apoptosis related protein) after long time point 24 or 48...

22 February 2015 8,257 4 View

Other than MTT is their any direct sensitive assay to observe proliferative properties of anticancer drugs screening?

I want to check the proliferative effects of some anticancer drugs (more than 50). I observe through CCK-8 or MTT assay. What is the best assay to check their proliferative properties other than MTT.

22 February 2015 2,046 13 View

Is there any extra care to culture 1975 cell line?

I got this cell line from 2 different sources. It primarily belongs to ATCC and culture according to ATCC guideline. But in both cases cells die after 2nd passage. I am using 10% FBS in RPMI media.

07 October 2014 7,318 3 View

To isolate single lung interstitial cells from mice lung fibrosis models, which collagenase should be used?

I read some papers in which they used sigma C2139 (which is collagenase VIII) while some papers reported colagenase III. Can I use any collagenase to isolate interstitial cells for flow cytometry...

07 April 2014 9,345 2 View

For cytokine detection, is there any special treatment or method to detect IL-1B and IL-6 by using the dry method of western blot?

We are detecting different samples, tissues and cells, to detect inflammatory cytokines. We are getting good results for B-actin, NF-KB and IL-12 but after trying so many times we were unable to...

03 April 2013 8,879 16 View

I want to work on a mouse lung cancer cell line for the inflammatory response. Any suggestion which mouse(Not human) lung cancer cell line is the better choice?

I previously worked on the human lung cancer cell line A549 in BALB/c nude mice. Now I want to find out about the inflammatory response of some chemotherapeutic agent in BALB/c mouse by using a...

14 January 2013 9,475 2 View