Heard some researchers have always purchased RPMI (no L-glutamine), and only added L-glutamine when preparing the complete cell culture media. Wondering if this has to do with the 'freshness'/'stability' of L-gluatmine in the cell media? Or whether the concentration of L-glutamine might be diluted if additional components are added to the media that already has L-glutamine?
Sharon: You are right that the glutamine difference was for freshness/stability as the amino acid tend to degrade over time. We just use alpha-MEM containing nucleosides and added non-essential amino acids for HepG2, which growth beautifully.
Thanks for your reply Ke-Wei Zhao. Do you know how long the glutamine is stable in RPMI such that it will not be toxic to the cells? We are thinking to use the RPMI (already with Glutamine) for cell culture since it was purchased by accident.
Dear Sharon,
we are always purchasing medium with everything already inside beside FCS and PenStrep. We never had any problem and our cells are feeling fine ;-)
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