What parameters of quality control would I have to consider for antimicrobial toothpaste?

Hello, my name is Bianca and as a project for university I have assigned the manufacturing of antimicrobial toothpaste- quality control department. I tried to research online but I could not find...

17 January 2021 918 3 View

Would anyone have a sample survey questionnaire for an AHP-TOPSIS study?

I am currently working on my undergraduate thesis project and we are trying to compare the AHP method with the AHP-TOPSIS method. I have already found the format for an AHP survey questionnaire,...

03 January 2021 7,213 3 View

Would anyone have an explanation of the occurrence of this phenomenon in the LS-Dyna simulation in the drum seaming roll process?

Hello, I am simulating in Ls-Dyna a drum seaming process that consists of closing a drum, ie the lid with the body using two additional tools (figure 1). The tools are set as rigid shells and the...

26 August 2019 4,808 1 View

How to write a methodological paper?

Dear all, I would like to write a methodological paper. Notably, I would like to explain how a particular methodology can be used to study problems related to a specific field of studies (i.e....

21 October 2018 2,254 22 View

Could you suggest must-read literature of socio-ecological systems?

Hi everyone, I might be approaching the field of socio-ecological systems to write the final paper of my PhD. I would like to get an idea of a super MUST-READ literature to get an overview of the...

04 April 2018 7,009 26 View

Has anyone one used (snap)frozen tissue samples in a ProcartaPlex Multiplex Immunoassay?

The guidelines states that it is suitable for serum, plasma and cell cultured lysates. Theoretically can you use animal tissue and make a tissue lysate?

21 September 2017 8,408 2 View

Regarding the use of water samples, what other testing methods can be used besides Coliform test for water that are more efficient and cost-friendly?

19 April 2017 5,018 3 View

Missing concentration-dependend effect of IL-2 concentration by TERB and NAD on human PBMC?

I have some questions regarding the results of my experiments. I need inspiration and ideas to discuss these results. Hopefully someone can help me to explain this. A short introduction of my...

21 January 2017 8,818 3 View

Is anyone aware of a described gene sequence for feline Angiotensin II?

I am trying to design an RT-qPCR primer for Angiotensin II in cats.

15 August 2016 3,872 3 View

Ways to produce aluminum powder from aluminum bar?

I am looking for ways to produce powder from an aluminum bar. I can't find an available grinder/mill, so I am looking for an alternative. I saw a method wherein a steel plate and a steel bar were...

07 June 2016 3,949 15 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View