28S ribosomal RNA probes for pulldown?

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What is a research topic that Honours High Performance and Honours Biokinetics students can work on togther?

Im asking this to get understanding of what the experts think is research topics that is easy for me to do when i get to honours .A topic that needs research to be done .Im writing a concept and...

03 May 2024 3,006 1 View

How does the lack of trained personnel and expertise in blood banking and transfusion affect the quality and reliability of blood transfusion service?

how does the lack of trained personnel and expertise in blood banking and transfusion affect the quality and reliability of blood transfusion services in Western Kenya? In regard to personnel,...

10 April 2024 2,137 1 View

How does quality control differ from quality assurance?

specially in laboratories

03 September 2023 9,097 4 View

What is the body composition of a zebrafish larva with 96 hpf?

I only find adult zebrafish.

04 July 2023 7,326 1 View

Does anyone have the file "Hostile and Sexual Behavior Patterns of South American and Pacific Laridae" published by Moynihan in 1962?

Dear colleagues, Does anyone have the file "Hostile and Sexual Behavior Patterns of South American and Pacific Laridae" published by Moynihan in 1962? Thanks and Sincerely, Bianca Ribeiro

08 June 2023 2,264 0 View

What would be the best way to test for the oil degrading efficiency of fungi?

Good day! I'm an undergraduate student working on my senior thesis. I am studying the isolation and degradation of oil-degrading fungi sampled from the sands of an oil spill-affected coast. I have...

25 April 2023 2,501 1 View

Is there a way to track gene flow of a species using ancient DNA samples from fossils?

I am a university student with access to limited resources, and I am trying to conduct a study on illegally traded wildlife and its correlation with zoonotic incidents. Would it be possible to...

03 March 2023 1,472 2 View

Where can I find my researcher ID?

i need to know my researcher ID for WOS

18 January 2023 6,821 0 View

TMT-16plex samples can increase nLC pressure?

I’m running a batch of mouse tissue tagged with TMT-16plex. Samples were fractionated in Agilent after S-trap. They look really clean… All maintenance was done in nLC. Precolumn and analytical...

13 January 2023 1,313 0 View

Detaching human monocyte-derived macrophages?

Dear all, I´m working with human monocyte-derived macrophages and would like to analyse them by flow cytometry. After monocyte isolation I differentiate them with 100 ng/ml M-CSF for 6 days and...

15 November 2022 8,397 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View