Hi. I did a stupid mistake and saved my photos in .png format which results in colourless images (should be green). Is there any way to convert back? I tried some converterst and fiji program but no luck. In files you can see how it should look like (green) and how do i see it in .png format on my computer. Funny thing is if i open the ohotos with the fluorescence microscope it shows the photos as it should but i cant convert them with the microscope...
Dear Marko Klimez
normally it should work in ImageJ Fiji. Convert your image to 'Composite' and then using the 'channels tool' you should be able to change the colors as you wish.
you can look for more details here at the 'Make composite' section in this document- https://imagej.nih.gov/ij/docs/menus/image.html
best wishes
The color that you see on a fluorescence images are pseudocolors and you can assign any color you want. Open the image on ImageJ or FIJI and assign whatever color your fluorescence signal want to be.
Open the image on FIJI > Click Image tab > Click Color > Click Channels Tool (a pop up Channels window will opens)> Make sure Color is selected on the drop down list and click More > Click whatever color you want from the list that opens.
Now you can save it as color images.
Besides, always save images in a lossless format (TIFF) unlike the lossy formats like .png, etc. Once it is in a lossy format, even if you save the image to a lossless format later will not improve the image - so save it in the right format from the beginning.
Good luck!
@Sathya Srinivasan
Thank you! You saved me 3 weeks of additional work. It worked as described above.
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