What is the maximum number of reads per one run with NGS?

Does anybody know how many reads are usually generated with Illumina NextSeq? For sequencing plasmid library (100-150 bp per read), for example.

27 September 2020 799 2 View

Hello dear researchers, how do you remove the black foil from the vine nursery?

Hello, how do you remove the black foil from the vine nursery? Attached picture in the file. is there machine way or by hand?

04 September 2020 3,566 2 View

Glioblastoma GL-261 cell line not growing s.c?

Hi has anyone had experiance of GL-261 not growing subcutaneously? I have inoculated 10e6 cells in C57BL/6J female mice and the tumors became palpable after a week cca but have subsequently...

19 February 2020 7,543 2 View

Microplastic extraction from fish guts using soluble filters?

Hi there, I want to isolate microplastic particles from fish guts and water samples. My initial idea was to extract guts from selected specimens and then dissolve the whole guts using 10% KOH...

12 February 2020 4,467 8 View

Why are my glucose levels decreasing very rapidly?

Hello, I'm conducting an IPGTT experiment on WT and KO mice (mice which we have shown to have improved glucose tolerance) and I have a serious issue of glucose levels plummeting after 30-60min....

28 September 2018 962 4 View

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10 December 2015 1,475 1 View

How can I calculate deflection of steel fiber reinforced concrete beams?

How can I calculate deflection of SFRC beams (ordinary and high strength)? Is there ACI or other proposals?

11 May 2015 4,977 14 View

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I need approximately 100 meters of ceramic cord (0,5mm). I need it for attaching extensometer to the tensile specimen when conducting tensile test at elevated temperatures.

10 November 2014 4,572 3 View

What is the best bacterial vector for large inserts (around 50 kb)?

Is it possible to insert 50 Kb of DNA to a bacterial vector and successfully maintain and amplify it in bacteria? If so, what would be the best vector?

12 January 2014 8,832 2 View

Where could I find complete interviews of some convicted Western jihadists?

I am NOT looking for studies on the subject, but actual interviews.

01 January 1970 5,977 2 View

Changes in the spatial distribution of widespread mosses under climate change?

Are there any publications or own observations on how the spatial distribution of widespread moss species such as Pleurozium schreberi, Pseudoscleropodium purum or Hypnum cupressiforme changes...

12 November 2020 6,758 6 View

Can anyone recommend a method of isolating terpenes (including sesquiterpenes) from dried hemp?

Can anyone recommend a method of isolating terpenes (including sesquiterpenes) from dried hemp? The idea is to separate the cannabinoids fraction from the terpenic fraction. We will use...

29 September 2020 1,267 3 View

What is the mechanism of Burstein - Moss blue shift in heavily doped p-type INDIRECT BAND semiconductor?

What is the mechanism of Burstein - Moss blue shift in heavily doped p-type INDIRECT BAND semiconductor? Initially in p - type semiconductor, the Fermi level is closer to valence band. Due to...

20 August 2020 289 4 View

Self polymerization of Sesquiterpene Lactones after storage?

I have dehydroleucodine (DHL) and parthenolide (PTL) in powder form. This powder was left at -4C for a year. When the powder is redissolved in ethyl acetate or methylene chloride it rapidly forms...

02 August 2020 3,173 4 View

What is the best technique / extraction method for extract sesquiterpene coumarins from oligo-gum resins of Ferula asafoetida ?

Phyto-chemicals such as Conferone, Samarcadin, Bdrakemin Farnesiferol A, Fernesiferol C and Galbanic acid can be isolated from Ferula asafoetida. suggest proper extraction methods and solvent to...

22 May 2020 6,350 6 View

Which species of moss is most suitable for combating air pollution ?

As Indoor air pollution and increased carbon dioxide in the environment causing health hazard not only to humen but also whole ecosystem, for this strong environmental policies are needed to be...

20 January 2020 3,693 6 View

How to measure atmospheric CO2 with GC-TCD ?

Hi all, actually im trying to develop a method to measure moss microbial biomass by substrate-induced respiration (the production of Co2). My problem is when I try to do a blank by injecting air...

06 July 2019 9,078 3 View

Why bonds are breaking while relaxing a two dimensional sheet?

Hi everyone, My query might look silly but I am new to VASP. I am trying to reproduce few results for learning purpose. I took a two dimensional MoSSe sheet. I have added...

27 June 2019 6,500 3 View

Why bonds are breaking while relaxing a two dimensional sheet?

Hi everyone, My query might look silly but I am new to VASP. I am trying to reproduce few results for learning purpose. I took a two dimensional MoSSe sheet. I have added...

05 June 2019 2,370 4 View

How can I get, Handbook on Semiconductors Trevor Simpson Moss, Minko Balkanski, Cyril Hilsum, free download?

How can I get, Handbook on Semiconductors Trevor Simpson Moss, Minko Balkanski, Cyril Hilsum , free download?

04 May 2019 656 2 View