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Questions related from Marko Klimez
In my cell culture i have GFP positive and negative cells. I would like to know if there is a difference in cell proliferation rate in positive compared to negative cells without sorting the...
08 May 2022 8,433 3 View
I am doing a gene knockout. Target gene: TRIM58. TRIM58 consist of 6 Exons. I'm inserting SFFV Promotor + Turbo GFP in the middle of Exon 3 using Cas9+sgRNA RNPs (nucleofection, LONZA 4D, EO 100...
02 December 2021 8,769 1 View
i'm doing Knockout of gene TRIM58 in CD34+ cells which are then going through Erythropoiesis to see if the KO has an impact on the enucleation process. So i start with Hematopoietic stemm cells...
21 November 2021 6,516 0 View
Hi. I did a stupid mistake and saved my photos in .png format which results in colourless images (should be green). Is there any way to convert back? I tried some converterst and fiji program but...
18 August 2021 6,071 3 View
Hello. I have 6 different primers for 2 different DNAs. The Forward and reverse primers have different lenght and GC%. I was wondering what T would you suggest or maybe should i design better the...
01 January 1970 3,116 3 View
Im researching the role of gene TRIM58 in enucleation process in hematophoeisis. I did already a knock-out of TRIM58 but i would like to do a cell line where i overexpress it too. What would be...
01 January 1970 757 5 View
