Hadas Gil

4 Questions 2 Answers 0 Followers

Questions related from Hadas Gil

Can cy5 be fixed using formaldehyde ?

Hello I am planning to deliver a cy5-labeled drug to mice, and then check its distribution in different organs. In case I wish to preserve the tissues for later research, will fixation (using 4%...

13 November 2023 4,825 1 View

FIXATION TIME before tissue slicing for paraffin infiltration - how much time?

Hi I need to make paraffin blocks to my murine hearts (extracted from 3 month old mice). Following extraction the hearts were held in 4% formaldehyde and 2 days later transferred to 70% EtoH. I...

20 April 2020 7,471 0 View

Endothelial cells present in fibroblasts primary culture from mouse?

Hi, I isolated cardiac fibroblasts from a mouse and cultured them in P.1. After mRNA extraction I saw high expression levels of CD146 gene, so I wonder if at that point it's possible that ECs...

08 July 2019 5,218 3 View

Flow cytometry on fresh primary fibroblasts?

So... I am finding it difficult to perform flow cytometry analysis of fresh primary cell culture. The cells I'm supposed to be using are FRESH (2 hrs incubation max) primary fibroblasts, extracted...

01 January 1970 9,535 3 View