Fix and mounting media for eGFP...

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Plasmid disappears after glycerol stock?

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SEAP plasmid wanted...

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Freezer database software, how to manage cell samples in freezers?

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Help needed with compensation

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From primers to synthetic DNA duplex

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Empty vector control...

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I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

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Can anyone help with Schistosoma identification?

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IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

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How to clean up TEM grid with plasma cleaner?

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How we can measure Kemp % in wool without using of benzene?

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Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

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Can the intensity of this broad band in XRD mask other crystaline phases present?

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Nicola Lo Buono

signal in live cells is good...

and PPD can be a good antifade...but I haven't problems with fading with mAb signals...only with GFP...

formalin can be the answer (PFA contains methOH, if I well remember)...I'll try with 10% formalin next time...

tnx...

Bernard Distel

You might consider using the fixative mercuric chloride (5%) instead of the 10% formalin. This will avoid the bleaching of eGFP. The concern with this fixative is the disposal of the slide when you have completed analysis due to the mercury factor.