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Questions related from Nicola Lo Buono
I've a strange problem: I transformed E.Coli (DH5alpha strain), checked plasmid by PCR, grew them O.N. as mini culture for miniPrep, and freezed them as glycerol stock. Now, I took my glycerol...
01 January 2014 384 9 View
I'm looking for a plasmid (any backbone can be ok) encoding for SEAP (Soluble Embryonic Alkaline Phosphatase). I've tried to buy it from plasmid databases (addgene, etc) but my new department...
04 April 2013 8,795 1 View
Have you got a free and really powerful software for managing specimens in freezers? A php script with a mysql database, a free software with a clear GUI or something similar? We're full of books...
11 November 2012 1,749 5 View
Hi everybody! I'm a bit confused about compensation. The problem is: I've a mixed population of cells some positive for a CD, others not. I need to analyze their cell cycle (easy), but selecting...
05 May 2011 3,324 10 View
Hi everybody! I've some trouble with eGFP tagged protein and visualization on confocal microscopes. My chimera is a membrane protein and after fixinig expressing cells and mounting the cover...
11 November 2010 2,840 2 View
HI! I've transfected a cell line with a pcDNA engineered plasmid with my gene of interest and a pcDNA - empty vector - as control... Well, the mock cell line has a strange phenotype, different...
11 November 2010 1,091 0 View
Hi everybody! I'd like to add a tag to my cloned protein...but I've some issue to overcome. 1) I've no useful expression vectors with a suitable tag to use 2) in my plasmid there's not suitable...
11 November 2010 9,764 4 View
