FITC-dextran blood samples, should I keep them at -20°C or 4°C?

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Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Permanent Fluid temperature initialization in Ansys Fluent?

Dear researchers, I am coupling Design Optimization with ANSYS Fluent in Workbench 2020 R2. I can do the analysis. I am patching the initial Temperature values of the zones. However, the patch...

28 January 2021 4,265 2 View

Luciferase assay for miRNA-gene: How to obtain DNA (PCR product) from the mutated primer?

To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...

19 January 2021 8,568 3 View

Which peak should I consider when calculating ZIF-8's crystalline size from XRD?

Hello, Using the Scherrer's equation, one could calculate, roughly, the crystalline size of ZIF-8. However, I am confused as to which peak in the XRD pattern should be taken for the calculation?...

10 January 2021 8,633 3 View

How to couple Moldex 3D with MATLAB for design optimization of an injection mould?

Hello, I would like to use the software Moldex 3D for the design optimization of an injection mould. How can I couple Moldex 3D with a MATLAB code? Any help appreciated, I wish you all a happy...

09 January 2021 569 2 View

How to setup ANSYS Fluent for simulating the filling stage of an injection mould?

Hello, I am using ANSYS Fluent for simulating the filling stage of an injection mould. However, when comparing the results with those of specialized software, such as Moldex 3D, I found the...

07 January 2021 7,992 1 View

Which plants and trees have the best ability to prevent the entry of particulate matter (PM2.5 and PM1) and nanoparticles in indoor environments ?

In recent years our group has highlighted the importance of trees and plants in public and recreation parks in large mega cities. However, we are interested in developing "green shields" to be...

05 January 2021 6,500 3 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View

How to perform Potential Surface Energy Exploration (PES) calculations?

I would like to receive help, as I am trying to make an analysis of the ethanol PES, but the calculations are always giving error. I use the conda-forge version of xtb (* xtb version 6.3.2...

04 January 2021 602 2 View

How do you create a funnel plot in STATA to assess publication bias using proportions from the metaprop command (for a meta-analysis)??

Hi everyone! I am trying to use STATA to create a funnel plot to asses publication bias using proportions from studies for a large meta-analysis (having used the metaprop command). I"m having...

09 December 2020 6,096 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View