Paula da Silva Frost

5 Questions 1 Answers 0 Followers

Questions related from Paula da Silva Frost

P-syk probing in cell culture protein extracts?

Hi all, I need help. I have been trying to assess p-syk (65E4) in THP1 cell extracts unsuccessfully via western-blotting. Anyone has any tricks I should try? So far I have been using PVDF...

12 September 2023 1,277 1 View

How to fix a Western Blotting experiment of GAD65 showing a band at ~50kDa?

Hi all, I have just used mouse brain samples to investigate levels of GAD65 via western blotting (ab239372). I got very specific staining, but the band appeared to be in a different weight around...

06 October 2021 7,041 3 View

What is the protocol for tissue processing (immunohistochemistry) in mice already expressing GFP in your protein of interest?

We have CX3CR1-GFP expressing mice and want to collect intestine tissue for IHC analysis. I am not sure how to process the tissue as too much time in PFA may quench the fluorescence. Anyone out...

13 May 2021 3,676 3 View

How ex vivo TEER measurements of the intestines change with glucose present?

Hi everyone, I have a technical question. I am performing some TEER measurements of ex vivo intestine tissues on ussing chambers. The problem is as I measured current I kept the lumen side with...

16 January 2020 3,287 0 View

FITC-dextran blood samples, should I keep them at -20°C or 4°C?

I have used FITC-dextran in my mice via gavage, and collected the blood samples to run together. Should I keep the samples at -20C or 4C? If I freeze my FITC can that affect the resulting...

01 August 2019 305 2 View