First strand cDNA synthesis from miRNA using polyadenylation will also tag mRNAs?

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What is the principle/mechanism behind aging of carbon (graphite) containing refractory mix for isostatically pressed refractories?

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23 July 2024 3,205 0 View

What binder would be the best choice to modify glassy carbon electrode with a 2D Material like Graphene or MXene?

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23 July 2024 5,776 2 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

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21 July 2024 7,284 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

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19 July 2024 148 6 View

What is the future scope of acoustic emission?

17 July 2024 1,510 1 View

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12 July 2024 3,745 2 View

Why can't I detect the plasmon resonance angle with water?

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10 July 2024 4,719 3 View

Does Nature Scientific Reports waive open access fee for industry authors?

I came across the Green Building and Sustainable Architecture collection under Nature Scientific Reports some weeks ago. https://www.nature.com/collections/gajghaebce The special issue/collection...

10 July 2024 5,533 1 View

Can I do Parallel Analysis for Principal Axis Factoring Method?

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30 June 2024 1,813 2 View

How can we generate topology file for water and helium system?

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24 June 2024 1,242 0 View

Anyone having idea about VN primer for miRNA primer design ?

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05 August 2024 2,116 3 View

The Optimal Experimental Approach for Gene Function Analysis?

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04 August 2024 6,265 1 View

What is the relationship between protein structure and N or C terminal tagging choosing?

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28 July 2024 366 3 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

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27 July 2024 6,920 1 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

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23 July 2024 1,329 5 View

How to transfect circular mRNA to T cells?

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21 July 2024 4,322 1 View

What happens in snapTotal- seq?

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14 July 2024 8,482 3 View

The criterion about maximally localized wannier function?

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13 July 2024 4,608 0 View

Stable cell line generation: GOI is detected by qPCR but protein is not expressed?

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11 July 2024 4,812 5 View

Gayatri Gandhi

Hi, no mRNA wont get polyadenylated, U can try ThermoFisher or Agilent

Abdul Ghani

Yes, there may be the chances for mRNA polyadenylation, so you need to design miRNA specific primer which only amplify your target. The best way for miRNA profiling is stem loop qRT-PCR. As the RT-PCR is highly sensitive so you should design your primer more specific.