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Questions related from Garima Jain
Have you ever tried RNA isolation from frozen plasma or serum samples? What concentration range you usually get? Would appreciate if anyone can recommend a protocol?
22 August 2024 5,289 1 View
I am trying to make cDNA from miRNA for qPCR. I am planning to go for kits. Most of them uses polyadenylation. I wonder if mRNA also gets adenylate with the kit protocol? also which kit is best...
25 September 2021 8,817 2 View
Which SYBR Green master mix (catalogue number?) is compatible with TaqMan™ Advanced miRNA cDNA Synthesis Kit (Catalog number: 4440040)? What alternative kits can used for miRNA --> cDNA synthesis?
25 September 2021 9,038 1 View
I have tissue biopsy punches stored in trizol at -80. Can anyone share the protocol to isolate RNA from such samples. Is there a possibility to isolate DNA, RNA and protein in same sample with trizol?
12 August 2021 1,184 3 View
In cancer biomarker identification most of the studies uses serum, EVs, or plasma as as source of miRNA isolation and identification. Why whole blood is not used as a source? Whole blood miRNA...
01 January 1970 1,033 3 View
