Is there a specific human fibroblast marker from patients with Idiopathic Pulmonary Fibrosis that murine fibroblasts isolated from the bleomycin model do not express? Would there be any suggestion of primers to identify a population of human fibroblasts in a co-culture with a mixture of human and murine fibroblasts in vitro? by PCR or IHC maybe?
Can anybody help me?
Thanks a lot for the help!
Dear FSP1 is an improved marker for lung fibroblasts that could be useful .
As widely accepted, alpha smooth muscle actin (alpha-SMA) is expressed abundantly in mesenchymal cells including activated fibroblasts (myofibroblasts). By striking contrast to alpha-SMA, FAP (fibroblast-activating protein) is exclusively expressed in myofibroblasts.
I raise all the fibroblast markers as far as I know;
alpha-SMA, asporin, collagen 11-a1 (COL11A1), fibroblast-activating protein (FAP), PDGFR alpha/beta, podoplanin, SPARC, S100A4, tenascin-C, microfibrillar-associated protein 5 (MFAP5), monocarboxylate transporter 4 (MCT4), MMPs, vimentin
Hey Remo, could you please share your protocol of isolating and culturing mouse lung fibroblast?
Dear Nupur Aggarwal, please find enclosed the article with the protocol that we perform in our lab.
Enjoy!
Remo
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