Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

How to run a statistical comparison of same group and 2 conditions in fMRI?

Hi everyone, I have some doubts about which statistical test to apply in order to test the probability of eventA to happen at the same time of non-eventB. Basically I have my fMRI group with...

14 September 2020 1,887 3 View

What is the best way to approach building a custom polypeptide?

I am trying to build a custom poly peptide that is ~50 amino acids in length, and it will ideally have a charge of ~+4 at pH 7. I can get the positively charged attributes from incorporating a...

08 December 2019 3,597 1 View

Olsen method, problem with calibration curve. How to fix?

Dear all, we are trying for the first time to perform the quantification of P with the Olsen method. We had followed the protocol proposed by Guide to laboratory establishment for plant nutrient...

20 September 2019 1,116 4 View

Whole Blood Over Recovering Compared to Blood Plasma?

My Immunoassay is giving higher analyte concentrations for the same sample before plasma separation vs after plasma separation. For instance, I run my assay with whole blood, and I get a reading,...

10 July 2019 7,391 3 View

Is there a valid and reliable scale for measuring the quality of student-teacher relationships from the student perspective?

Is there a valid and reliable scale for measuring the quality of student-teacher relationships from the student perspective, that has been used with primary age children? Is there any research to...

04 June 2019 10,015 1 View

PCR: lower bands. How overcome high Tm?

I am trying to amplify the luminal domain of TRAP alpha subunit (ER), but probably TM is too high. I performed PCR with two different pairs of primers but I get bands below 500 bp (see picture),...

12 July 2017 1,156 10 View

Why my frequency calculation aborted?

Hello, I am trying to running a frequency calculation with gaussian for a dimer that is made by benzene and bromine fluoride at T-shape. After finding a stationary point in the optimization, the...

06 March 2017 6,071 2 View

Can you suggest an algorithm for the integration of miRNA and mRNA expression data (RNA-seq) in a time dependent manner?

Hi! I have 5 time points for mice development and I have miRNA and mRNA expression data (RNA-seq) for each time point. I would like to integrate these expression profiles in a dynamic way. I know...

30 June 2016 8,444 4 View

How to dilute human Fc block for immunofluorescence?

Hello guys, I am a BSc student and my supervisor is currently on holiday and I forgot to ask about this. i have a human FC block from BD Pharmingen. It does not state the dilution i need to do for...

19 June 2016 4,827 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

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22 February 2021 9,932 11 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View