Has anybody a protocol to determine the AChE activity in larvae zebrafish brain?

Even following the instruction of Karnovsky and Roots (1964), I still do not know how long I should fix the larvae and in what fixative. Could anyone help me? Thank you!

14 January 2021 385 2 View

Women learning styles/ Women cognition development- Any suggestions for research based on Belenky's work?

I'm looking to expand my sources on the topic

10 December 2020 5,426 17 View

How can I get an homogeneus suspension of Bacillus subtilis?

I have different plates with different strains. The next step consists of doing a suspension of each microorganism. I do not have any problem with E. coli, S. aureus, etc, but I do with B....

29 January 2020 8,348 1 View

Flow cytometry -advance pipeline & compensation?

Hii! So I designed for my next experiments a quite complex panel and I have a few questions about how better proceed for treating my Abs and building my compensation. So I have a Fixable...

14 November 2019 6,590 3 View

How to avoid "U" shape RNA bands in LE agarose gels?

15 April 2019 9,668 2 View

Problems with biological indicator in autoclave, why?

I have problems with the new autoclave. We checked the sterilization process and realized that the biological indicator (B. stearothermophilus) grew after the sterilization. The technician put...

23 January 2019 9,796 3 View

No title

I obtain few astrocytes after the subculture step. Some astrocytes remain adhered to the culture dish surface after washing with PBS (Without Mg and Ca), incubating 15 min with PBS (Without Mg...

12 November 2018 4,172 2 View

Number of passages for the mouse choroid plexus cell line ECPC?

08 April 2018 8,872 1 View

How to connect routers to an existing network?

Hi! I have a network created with WiFi modules and I want to connect routers to that network so they can forward the messages to the corresponding module. If someone could give me some information...

31 August 2016 5,813 6 View

How to start implementing a VANET prototype?

Hello! I would like to implement a VANET prototype in an Arduino or something similiar and I don't know how to start. If someone could give me some advice on what do I need to start implementing...

13 June 2016 9,574 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View