5 Questions 11 Answers 0 Followers
Questions related from Alba Manresa Arraut
I am trying to optimize an in vitro BBB model to study lymphocyte migration from the blood stream into the CNS. In my set up I use a 24-well plate with Transwell inserts of 8µm pore-size. The...
06 August 2015 6,153 3 View
I would like to confirm that my lymphocytes are stocked on top of the membrane of the transwell insert after a migration assay. After the migration assay. I stained the transwell inserts with...
20 February 2015 7,206 7 View
I'd like to try a migration assay with matrigel-coated transwell inserts, does anyone have experience on that? I would appreciate tips on the matrigel concentration, T-cell concentration (of EL-4...
05 February 2015 6,208 2 View
Hi all, I am analyzing the myeloid cell populations in pancreas of NOD (non-obese diabetic) mice. My gating strategy for now is as follows: Gate for live CD45+ cells --> Select CD11b+ myeloid...
01 January 1970 6,655 5 View
Dear all, I have a panel with the following Abs: CD45, CD11b, F4/80, CD14, CD11c, Ly6C, Ly6G, CD163. I gate for CD45+CD11b+ for myeloid cells, and from there I gate neutrophils Ly6G+. My question...
01 January 1970 2,381 3 View