Alba Manresa Arraut

5 Questions 11 Answers 0 Followers

Questions related from Alba Manresa Arraut

How can you quantify the migrated EL-4 lymphocytes in a Transwell migration assay?

I am trying to optimize an in vitro BBB model to study lymphocyte migration from the blood stream into the CNS. In my set up I use a 24-well plate with Transwell inserts of 8µm pore-size. The...

06 August 2015 6,156 3 View

How can I improve immunostaining in transwell inserts?

I would like to confirm that my lymphocytes are stocked on top of the membrane of the transwell insert after a migration assay. After the migration assay. I stained the transwell inserts with...

20 February 2015 7,209 7 View

Has anyone tried to perform a migration assay with the lymphocyte cell line EL-4?

I'd like to try a migration assay with matrigel-coated transwell inserts, does anyone have experience on that? I would appreciate tips on the matrigel concentration, T-cell concentration (of EL-4...

05 February 2015 6,211 2 View

F4/80+CD11c+ macrophages in pancreas?

Hi all, I am analyzing the myeloid cell populations in pancreas of NOD (non-obese diabetic) mice. My gating strategy for now is as follows: Gate for live CD45+ cells --> Select CD11b+ myeloid...

01 January 1970 6,658 5 View

FACS panel for myeloid cells in mouse liver

Dear all, I have a panel with the following Abs: CD45, CD11b, F4/80, CD14, CD11c, Ly6C, Ly6G, CD163. I gate for CD45+CD11b+ for myeloid cells, and from there I gate neutrophils Ly6G+. My question...

01 January 1970 2,384 3 View