8 Questions 28 Answers 0 Followers
Questions related from Pieter Louwe
For an experiment I am thinking of sorting early apoptotic thymocytes. Does anyone have any experience with this ? I am a bit worried that the sorting process will turn my cells late...
19 February 2018 2,760 1 View
Hi Everyone, PKH26-PCL dye has been used to label macrophages/phagocytes (http://www.sigmaaldrich.com/catalog/product/sigma/pkh26pcl?lang=en®ion=GB) I have been thinking about using this...
09 November 2017 7,118 1 View
Hi, So, am new to the use of lentivirusses for transduction, so this might be a pretty basic question. I have made a lentivirus to express my protein of interest and an empty vector that lacks...
10 August 2017 2,755 1 View
Does anybody have some suggestions to stain sorted cells? For my experiment I have sorted cells based on a bunch of markers. After the sort I fixed the cells and the goal is to stain this...
20 February 2017 2,402 3 View
I have some mouse heart tissue embedded in OCT. Usually we take cryosections but we have decided to try and make thicker sections with a vibratome. I was wondering if anyone has experience making...
18 October 2016 1,820 2 View
Hi,Is anyone familiar with a stain for death cells in living zebrafish.More specifically I am looking for is a staining similar to Propidium Iodide (as in usable in live cells) but with a 405/647...
28 January 2016 3,063 1 View
I was wondering if anyone knows an antibody that can be used for staining microglia in zebrafish? I am familiar with 4c4 and L-plastin antibodies, but these are not perfect for me. Is anyone...
15 December 2015 7,598 9 View
I am trying to remove small(yet brightly fluorescent) specks from a 3d image. These specks are very hard to get rid of using conventional filtering and are interfering with my subsequent image...
07 October 2015 1,985 3 View
