Has anybody a protocol to determine the AChE activity in larvae zebrafish brain?

Even following the instruction of Karnovsky and Roots (1964), I still do not know how long I should fix the larvae and in what fixative. Could anyone help me? Thank you!

14 January 2021 385 2 View

Women learning styles/ Women cognition development- Any suggestions for research based on Belenky's work?

I'm looking to expand my sources on the topic

10 December 2020 5,426 17 View

How can I get an homogeneus suspension of Bacillus subtilis?

I have different plates with different strains. The next step consists of doing a suspension of each microorganism. I do not have any problem with E. coli, S. aureus, etc, but I do with B....

29 January 2020 8,348 1 View

Flow cytometry -advance pipeline & compensation?

Hii! So I designed for my next experiments a quite complex panel and I have a few questions about how better proceed for treating my Abs and building my compensation. So I have a Fixable...

14 November 2019 6,590 3 View

How to avoid "U" shape RNA bands in LE agarose gels?

15 April 2019 9,668 2 View

Problems with biological indicator in autoclave, why?

I have problems with the new autoclave. We checked the sterilization process and realized that the biological indicator (B. stearothermophilus) grew after the sterilization. The technician put...

23 January 2019 9,796 3 View

No title

I obtain few astrocytes after the subculture step. Some astrocytes remain adhered to the culture dish surface after washing with PBS (Without Mg and Ca), incubating 15 min with PBS (Without Mg...

12 November 2018 4,172 2 View

Number of passages for the mouse choroid plexus cell line ECPC?

08 April 2018 8,872 1 View

How to connect routers to an existing network?

Hi! I have a network created with WiFi modules and I want to connect routers to that network so they can forward the messages to the corresponding module. If someone could give me some information...

31 August 2016 5,813 6 View

How to start implementing a VANET prototype?

Hello! I would like to implement a VANET prototype in an Arduino or something similiar and I don't know how to start. If someone could give me some advice on what do I need to start implementing...

13 June 2016 9,574 3 View

What Research is yet left in the field of Automation and Unemployment?

I have been through a number of journals and Research Papers. I wanted to get an idea as to what new areas are still left to be researched in "Will Automation lead to unemployment or it will lead...

09 February 2021 9,332 4 View

What are the ways to test the knowledge level of agricultural labourer about their day to day work?

what kind of test can I do for testing the level of knowledge about their daily operation?

03 February 2021 7,583 10 View

Are there existing studies in economic sociology that have applied Kalyan Sanyal's theoretical framework as a methodological basis?

To be precise, I am looking for studies on informal labour that have taken on board Sanyal's category of the Need Economy and his 'Politics of Exclusion' in studying the political-economy of...

26 January 2021 4,219 3 View

Is it a feasible way to model CO2 emissions Mathematically?

I am working on the Environmental and Social indicators. So the easiest way is to get the solution is to go for any LCA tool;But my doubt is ,is it possible to mathematically model the Emissions...

17 November 2020 8,492 3 View

Structural equation modelling or multiple regression?

Hi all, I am currently building a proposal for my master's research and am a little confused about the best statistical method of analysis to employ. I'm researching the mediating effect of...

08 October 2020 2,551 7 View

What model/theoretical framework could I use?

05 September 2020 624 20 View

What will be the best methodology to quantify Demographic Dividend and Skill labour of any country?

Most of the researchers used panel data to show the impacts of demographic dividend. But for individual country where divisional or district data over the year are not available, what methodology...

29 July 2020 6,733 1 View

Outlier detection in labor economics?

I was curious about the possible Machine Learning methods or approaches for outlier (anomaly) detection in labor economics. I tried to search for this matter, but could not find any pertinent...

20 July 2020 1,632 3 View

How do you interpreted a positive/negative insignificant coefficient?

In my case: Effect of a minimum wage (independent) on labor hours per week (dependent) Full Time Workers: -1.359, but insignificant Part Time Workers: 2.131, also insignificant Could you still...

17 June 2020 2,155 2 View

Whether agriculture in India is robust enough to contribute substantially so as to minimise the expected decline in GDP in post Corona scenario??

Before the Corona pandemic, agriculture sector in India contributed around 15 percent to the total GDP growth. In the present scenario, service and industrial sectors are bound to pull down the...

27 April 2020 9,608 11 View