What statistical analysis method should I use with multivariate categorical data?

Hi, I'm struggling quite a bit, Hope someone can help. My research question is roughly to what extent (and what) characteristics of murder and manslaughter cases are related. The data consists...

05 October 2020 3,958 8 View

Problems with Bovine Fibrin Clots?

Hi I am using Fibrinogen (10mg/ml) and Thrombin (1000U/ml) to form fibrin clots. I found that after getting good cross linking initially the clots dissolve to liquid if left in incubator...

26 May 2016 9,416 4 View

When I added the interaction terms, the previously identified significant variables became insignificant?

I'm running a logistic regression test. On my first test, I've identified 6/7 significant explanatory variables. I then ran another logistic regression test with all the interaction terms. The...

05 April 2016 7,655 3 View

Anyone have a good methodology for Agar Gel for adding on top of a cell culture dish (and that allows you to pour/pipette as a thin layer)?

07 February 2016 7,189 1 View

For Fibrin Matrices is >2000 NIH/ml best - or can one use 600-2000NIH/ml in order to produce 1000U/ml concentration (Thrombin from Bovine Plasma)?

- used for making matrices to seed neonatal fibroblasts.

02 February 2016 8,338 1 View

What is the ultimate purpose of Ecology, and what do we hope to achieve in advancing the field?

01 February 2016 9,757 25 View

In what way can spirituality be experientially incorporated into Early Years Education in the UK?

21 May 2015 7,674 4 View

How can I find out the interaction of an osteoarthritis patient's DNA with Vitamin B complex?

I have osteoarthritis patient DNA. Now I want to study the interaction of the DNA with Vitamin B complex. What is the procedure to do so? What types of materials and methods are used to study this...

26 November 2014 1,981 2 View

How can I identify the subtypes of HLAB27 when I used Real-TM PCR which show qualitative results?

I used Real-TM PCR which shows qualitative analysis for identification of HLA B27 after DNA extraction from patient blood sample. The analysis show me whether it is positive or negative. But I...

12 October 2014 3,675 1 View

What is the process of calculating Rf factor from SDS-PAGE after protein extraction?

protein extraction

08 October 2014 7,028 2 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

Why do I see variability in mRNA expression in qPCR of the same gene (ubiquitin)?

Hi everyone! I use HaCaT cells and i transfect them with siRNA. After 48 h I extract proteins with Laemmli and RNA with trizol. I proceed with western blot to check proteins levels (and they are...

26 January 2021 5,738 3 View

How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

What Lysis for frozen tissus for nuclic acid extraction (Maxwell plus RSC FFPE kit) ?

Hello, I have a technical question for you. For RNA and DNA extraction, we use Quiagen kit with Trizol/thiocyanate de guanidine lysis. However, it's a bit long and we wanted to improve our...

14 December 2020 7,596 1 View

Most recent, reliable, optimized methods to extract both RNA and protein from flash-frozen brain tissue?

I have flash-frozen brains and am looking to extract RNA for RNA-seq, and protein for western blotting. I've seen other questions related to this...

10 December 2020 6,294 0 View

How to improve DNA extraction using TRIzol and chloroform?

02 December 2020 1,919 1 View

Trizol samples wont freeze. Can it cause problems?

Hi, everyone. I recently lysated cell culture with Trizol reagent, and I put my samples in -20C. But, some of my samples wont freeze, and I can see two separete phase appearing in them. I'm awaer,...

06 November 2020 10,002 1 View

I am trying to isolate the RNA by trizole method, the nanodrop gives the A260/230 ration above 2.0, however no bands appear when I run on gel in TBE?

Any suggestion.

20 October 2020 8,143 4 View

What is the better method for RNA/protein extraction: Trizol or Methanol/Chloroform?

We want to do transcriptome and phosphoproteome analyses of the same sample and have limited material. So a simultaneous extraction method for both (RNA and protein), such as Trizol or...

18 October 2020 1,708 1 View