I am culturing Expi293 cells where i have stored many vials and grown till passage 10 without any trouble. But when i tried to revive the cells cells the cell growth is perfect till 3 days and on fourth day the cells will be dead and media becoming turbid.

I have checked for Mycoplasma contamination but it was negative and i have added antibiotic and it doenst make any difference. We changed everything and the problem still persists. I am doubting the stored vials but these vials were growing fine when i stored.

has anyone faced the same problem and anyone has any remedy for the same?

#Expi293 #Contamination

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