I am culturing Expi293 cells where i have stored many vials and grown till passage 10 without any trouble. But when i tried to revive the cells cells the cell growth is perfect till 3 days and on fourth day the cells will be dead and media becoming turbid.
I have checked for Mycoplasma contamination but it was negative and i have added antibiotic and it doenst make any difference. We changed everything and the problem still persists. I am doubting the stored vials but these vials were growing fine when i stored.
has anyone faced the same problem and anyone has any remedy for the same?
#Expi293 #Contamination