Shankar Anand

4 Questions 7 Answers 0 Followers

Questions related from Shankar Anand

Does any one used tandem copies of NF-AT/AP-1 consensus binding sites upstream of IL2 promoter?

what is the significance of tandem copies (6 copies in tandem) of NF-AT/AP-1 consensus binding regions upstream of the IL2 promoter? although there are three binding sites that naturally occur for...

25 January 2021 8,237 0 View

How to avoid transgene silencing in Mammalian Cells?

Hello, Does any one faced transgene silencing after transfection? I have been trying to get good expression from a recombinant antibody molecules cloned in pUC based vector and transfected in CHO...

20 February 2020 3,043 7 View

Have anyone tried Chlorine Dioxide Based Fumigation?

Did anyone used Chlorine dioxide based fumigation or disinfection for Biosafety level 2 laboratories? If so what is its effectiveness when compared to Hydrogen peroxide based...

12 December 2019 8,292 0 View

Expi293F cell death and Expi293 expression media turnning turbid.

I am culturing Expi293 cells where i have stored many vials and grown till passage 10 without any trouble. But when i tried to revive the cells cells the cell growth is perfect till 3 days and on...

01 January 1970 7,113 3 View