Hi scientific community!
I'm Carlos and for a new experiment I need to prepare the Postgate Medium B which is used to grow sulfate-reducing bacteria. I'm guiding myself with this guide (https://bacmedia.dsmz.de/medium/63) which says:
"Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas atmosphere into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121 °C. Adjust pH of the complete medium to 6.8 - 7.0, if necessary"
I'm a little confuse with the part of the Hungate-type tubes. Is it really necessary to distribute the mix before the autoclave if I've already purged all the mix with N2 to maintain the medium anoxic?
On the other hand, this paper "Article Cultivation and detection of sulfate reducing bacteria (SRB)...
" says that the medium should be used right away to avoid deterioration. Does anyone have the experience of using the same made Postgate in a long period of time?Thank you very much!
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