In my opinion it will be very difficult to find circulating plasma cells in normal individuals, you may find some plasma cells in people with lymphocytosis check CBC for your donors before and include donor with high lymphocyte count.

Best wishes

Aziza

In my opinion as well as in my experience there are no pathophysiological reasons to find circulating plasma cells in PB of normal subjects. For this reason, observing in the PB even only an isolated PC during a microscopic review, it is mandatory initiating a diagnostic pathway to detect a possible misknown plasma cell dyscrasia.

Anyway, considering that the plasma cells malignancy is due to their clonality, the “normal” correspondent of clonal PC are the not-clonal PC which can be observed more easily not in normal peoples but those having inflammatory or infectious diseases (so-called reactive PC).

In normal person detection of plasma cell as an reactive cell especially in infective or inflammatory process is possible

Thank you all for your helpful answers. I could successfully isolate enough magnetically labeled CD138+ plasma cells from healthy donors to perform my following experiments, though the number of the cells were quite limited.

Hi Milad,

Have you successfully isolated plasma cells from peripheral blood cells of healthy individuals previously? It would be interesting to know.

Thanks.

It would be interesting to know if PC were isolated from all tested healthy individuals as well as the amount of PC in PB.