6 Questions 14 Answers 0 Followers
Questions related from Milad Moloudizargari
I am doing a NK cytotoxicity assay using the NK-92 cells line and K562 target cells. I prelabel my target cells with CFSE for 20 minutes. Then I co-culture the NK cells and the labeled target...
12 April 2019 6,007 2 View
Has anyone performed a peripheral blood mononuclear cell (PBMC) cytotoxicity assay against K562? I am about to do this as a step prior to NK cell isolation and specific NK cytotoxicity assay with...
09 October 2018 1,639 1 View
I need two detailed and reliable lab protocols one for assessing NK cytotoxicity and one for NK degranulation assay using flow cytometry. Is NK cytotoxicity done only in co-culture with K562 or...
18 February 2018 3,002 12 View
I have a couple of questions regarding primary NK cell isolation from human peripheral blood: What is the practical yield of NK cell isolation from human blood? (for instance using the Miltenyi...
04 January 2018 683 3 View
What are the most reliable and scientific online/offline veterinary databases/softwares with organised guidelines and protocols which could help clinicians to reach the best diagnosis and...
11 September 2016 8,459 11 View
Has anyone performed the optimal compensation of FITC-conjugated annexin V and Propidium iodide in apoptosis studies with flow cytometry? I think my dot plot is either under or...
01 January 1970 8,491 3 View
