Milad Moloudizargari

10 Questions 32 Answers 0 Followers

Questions related from Milad Moloudizargari

Why are my low control (K562 ONLY) cells dying in the NK cytotoxicity assay?

I am doing a NK cytotoxicity assay using the NK-92 cells line and K562 target cells. I prelabel my target cells with CFSE for 20 minutes. Then I co-culture the NK cells and the labeled target...

12 April 2019 6,060 2 View

Experienced with plasma cells and multiple myeloma?

I am about to isolate CD138+ plasma cells using a Miltenyi Biotec MACS kit from the PBMCs of healthy donors. Although I know that circulating plasma cells are scarce in normal individuals, I need...

17 November 2018 5,214 6 View

PBMC cytotoxicity vs. K562?

Has anyone performed a peripheral blood mononuclear cell (PBMC) cytotoxicity assay against K562? I am about to do this as a step prior to NK cell isolation and specific NK cytotoxicity assay with...

09 October 2018 1,675 1 View

Protocols for NK cytotoxicity and degranulation?

I need two detailed and reliable lab protocols one for assessing NK cytotoxicity and one for NK degranulation assay using flow cytometry. Is NK cytotoxicity done only in co-culture with K562 or...

18 February 2018 3,064 12 View

Experienced in NK cell isolation and culture?

I have a couple of questions regarding primary NK cell isolation from human peripheral blood: What is the practical yield of NK cell isolation from human blood? (for instance using the Miltenyi...

04 January 2018 737 3 View

What are the most reliable and scientific online/offline veterinary sources?

What are the most reliable and scientific online/offline veterinary databases/softwares with organised guidelines and protocols which could help clinicians to reach the best diagnosis and...

11 September 2016 8,510 11 View

Can anyone give me a reliable LTT protocol?

Can anyone give me a reliable LTT (Lymphocyte Transformation Test) protocol to be used in diagnostics?

03 August 2016 8,669 4 View

Has anyone performed an LCA (Left Coronary Artery) ligation procedure on rat?

As you may possibly know, LCA ligation is a surgical approach used to induce ischemia in heart for ischemic/perfusion studies. I`m about to follow this method to start a new project, however I`m...

11 April 2014 9,162 17 View

Has anybody worked with an air pouch in vivo inflammation and angiogenesis model?

I`m experiencing trouble recollecting the inflammatory exudate induced by carrageenan after 6 days. I have to collect the inflammatory exudate in order to evaluate its volume and the infiltrated...

06 February 2014 9,735 18 View

Has anyone performed the optimal compensation of FITC-conjugated annexin V and Propidium iodide?

Has anyone performed the optimal compensation of FITC-conjugated annexin V and Propidium iodide in apoptosis studies with flow cytometry? I think my dot plot is either under or...

01 January 1970 8,533 3 View