Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Picrosirius red staining without picric acid?

I would like to perform picrosirius red staining but we cannot use picric acid anymore. Do anyone have already test other acid like citric acid instead? Thanks a lot

08 September 2020 2,794 2 View

Dopamine transporter antibody?

I am looking for an antibody against dopamine transporter suitable for WB. I have tried a couple of antibodies but I obtained poor results. Can anyone help me?

18 May 2020 1,536 2 View

Can anybody help me with Boyden chamber invasion assay for HCT-116 cell lines?

I have a problems with Boyden chamber assay for HCT-116 cell lines, I did not see any stained cell in the membrane. I have had a success with other cell line, A549. Does anyone have any...

23 March 2020 7,119 5 View

How to make cell distribute evenly in 12-well plate?

I worked with cell culture 1 year ago, nearly I have a problem when I seeding in 12-well plate, more cells settles in the middle of well. I am trying with different 'shaking method'. I hope to get...

19 March 2020 9,999 3 View

Can anyone provide the Scoring algorithm for the UK SF-12 Version 1?

24 February 2020 1,653 2 View

Can anyone recommend any strong papers concerning the prevalence of PTSD and/or CPTSD in paramedics?

Dear all, I am in the early stages of undertaking research surrounding PTSD/CPTSD and the prevalence of these conditions in paramedics. Can anyone point me towards some recent research in this...

29 October 2019 8,117 1 View

What is the best way to isolate the full leukocyte fraction from mouse blood?

I would like both monocytes, lymfocytes and granulocytes to eventually stain and stick on a slide. I used lymphoprep before, but this only gives me the mononuclear cells. I would like the complete...

08 October 2019 1,717 3 View

Could someone guide me towards my hesitation to choose my tax law thesis topic?

Good afternoon from Peru! I'm currently working on my thesis (Tax Law field), but I can't seem to decide which topic I would like to explore, because I am really interested in this three...

20 June 2019 4,419 4 View

Why is my whole western blot gray?

I have attached a picture for my most recent western blot. My Beta-actin always shows up completely perfect but all other protein blots appear gray and messy. This has happened multiple times....

31 May 2019 5,364 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View