Dear Jenny Jiménez

You can use % for the relative quantification.

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Kind regards

AB Bayazid

Obviously you are not going to be able to quantify in terms of actual amount of the antigen, but you could standardize against some some standard condition and call that you're 100% standard. Even better might be if you prepared many aliquots of your "standard" so you are always using exactly the same amount each time and then all your experimental values will be some percent of that standard.

Hi, can you please provide more details on the analyte being measured and the manufacturer of the assay. The Instructions for Use (IFU) should assist.

Hi. It is a GMO detection kit for the PAT/PAT protein.

Here are the instructions: https://www.goldstandarddiagnostics.com/pub/media/productattachments/files/AID_-_027_PAT-pat_ELISA_kit.pdf.

Thanks to all of you for your comments.