How to make sure that ultrasonic lysis of E.coli is done completely?

What's the criteria of comolete lysis of E.coli when breaking them using ultrasonic? What phenomena can help to determine if the cell are broken completely? From smell, transparence of the lysis...

07 January 2021 1,911 3 View

What is the difference between "pesticides" and "insecticides"? Are they same?

What is the difference between "pesticides" and "insecticides" in English? Are they the equivalent replacement when used in text? I am an ecological researcher who particularly concerns the...

04 January 2021 6,230 37 View

Ungrounded CPW fed slot-ring patch antenna not behaving as an antenna in CST simulation?

Greetings everyone, I am attempting to model and simulate a slot-ring patch antenna fed by ungrounded CPW in CST, although the S11 is fine, the e-field and h-field monitors shows strange...

10 October 2020 2,228 2 View

Can we perform MOX/MSTFA derivatization in Eppendorf tubes?

Hi there! Recently I am planning to work on metabolomics using GC-MS, where derivatization is a crucial step. Here, I am wondering if that possible to derivatize extract samples with both MOX and...

24 September 2020 9,695 1 View

What is the right time course when using cycloheximide to inhibit protein synthesis?

I'm going to use cycloheximide to inhibit the synthesis of a membrane protein in HEK-293T cells, but I'm not sure the right time that needed to treat cells without influencing its viability. I...

21 September 2020 4,321 2 View

How to eliminate the warning of 'position not found when parsing line' during DAMASK simulation with ABAQUS/standard?

Hi, I am trying to use the ABAQUS/standard solver to simulate the single crystal deformation behaviour with DAMASK framework. The materials is BCC ferrite with hooke elasticity and phenopowerlaw...

10 December 2019 4,667 1 View

What is the yellow/brown salt crystal during protein crystallization?

During protein crystallization screening, we encountered several types of proteins grew yellow stick crystals with a reservoir containing 0.1 M BIS-TRIS pH 6.5, 2.0 M Ammonium sulfate. However,...

09 January 2019 2,188 1 View

Does anybody know any company manufacture cyclic olefin polymers: COP (except Zeonex/Zeon)?

Want to try COP from different vendors! Is there any one in China? Thanks!

24 April 2018 8,736 2 View

What is a quick and easy screen for visualizing the presence of gold nanoparticles?

Hi all, I am trying to design a hypothetical screen to find additional genes that are responsible for C. metallidurans' ability to reduce gold ions (Au(I) and Au(III)) to solid gold nanoparticles....

12 February 2018 7,511 2 View

One virus may have two different kinds of particle?

15 February 2017 676 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View