I know MGB can bind and stabilize the duplex, but it should be low in extinction coefficient and Max. Ab.(nm). Does anybody know the quenching efficiency of MGB comapred to QSY/BHQ? Foy quenching@long wavelength, does MGB still do well?
I want to train neural networks to evaluate the seismic performance of bridges, but the papers online are all based on their own databases and have not been published. Where can I find the...
29 March 2024 6,946 1 View
I am currently exploring the practical applications of random regression models in the dairy industry. Through these models, it is possible to derive coefficients from the lactation curve,...
08 January 2024 3,758 0 View
Comparing gravitational field to electromagnetic field, the charge has the same sex and the opposite sex, but there are two kinds of "force"? Is there any gravity and any repulsion? What if...
20 December 2023 4,961 21 View
Laurie J. Jones; Stephen T. Yue; Ching-Ying Cheung; Victoria L. Singer (1998). RNA Quantitation by Fluorescence-Based Solution Assay: RiboGreen Reagent Characterization. , 265(2), 368–374....
31 October 2023 168 0 View
I would like to know if the binding between extracellular vesicles (EVs) in serum and immunoglobulins is strong or not.
02 August 2023 6,316 1 View
Ryves D.B. (2006, 2009) proposed the use of formulas for calculating the Diatom Dissolution Index (DDI). However, determining the dominant species of diatoms and classifying them into levels (1-4)...
01 August 2023 1,610 3 View
During my time processing results curves, I frequently need to correct the baseline before peak fit, and intensity comparison. This thing happens to XRD, XPS, Raman, and many other data...
02 May 2023 9,586 3 View
I have the thickness data and dielectric tensor from ellipsometry
06 April 2023 7,770 2 View
Dear all, can you please help me with a quesiton in XRD phi scan? Let's say we have a cubic Si (111) substrate, and I choose (100) plane to do the phi scan, how many peaks can we expect to...
28 March 2023 2,633 3 View
This figure mainly portrays bifurcation curves for the transverse destabilization of various low-periodic cycles embedded in the synchronized chaotic attractor.
26 March 2023 8,891 2 View
The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
27 July 2024 8,507 0 View
I was performing quenching studies in solution state. While plotting the Stern-Volmer graph, which intensity should I use as initial intensity (I0). For example, I take 1 ml of 0.1 M fluorophore...
25 June 2024 5,385 1 View
Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...
17 June 2024 3,434 5 View
I want to ask about the response of the analyte in the EIS measurement. Bare shows a high Rct response as compared to material-modified electrodes. But when we modify the material + analyte, the...
02 June 2024 872 3 View
Hi Researchgate folks, Our lab has mouse FFPE tissue which has cells that express a TdTomato reporter as well has a FAM labeled drug as we routinely use to test the delivery of the drug to the...
15 April 2024 6,631 0 View
I have a fluorophore and its emission is quenched on adding analyte compound. The overlapping spectra of compound and analyte make UV-vis absorption analysis unreliable. The analyte mixture...
03 April 2024 7,913 1 View
Share the reference of number of moles of steam (practically / experimentally) required to quench one mole of carbon monoxide(g) ?
20 March 2024 8,315 0 View
I am currently working to optimise RNAscope multiplex V2. I have troubleshooted the issue with several tests. I have washed thoroughly with wash buffer even with their recommended guidelines of...
29 February 2024 4,764 0 View
I encountered the fluorescence enhancement in the DNA interaction process. Since the classical Stern-Volmer equation is used for the fluorescence quenching process, this equation cannot be used to...
16 February 2024 2,776 1 View
Can BHQ quench the fluorescence of FAM in the case shown below?
28 January 2024 6,181 1 View