I noticed so much inconsistency in the indirect ELISA for cell culture supernatant and also for purified MAbs. Although some clones follow serial dilution say 2-3 out of 8 clones on the same plate. I find it difficult to titrate dilution.
Is this entirely handling mistake or is there any other way of doing dilutions accurately?
Michael G. Weller
Did you filtrate or centrifuge all your samples and solutions properly? Often particles are the main cause for ELISA problems.
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Michael G. Weller
If you want to perform a serial dilution in the same plate, you should consider that careful (!) mixing after each step is crucial for a consistent result. The multi-channel pipette should be checked, too.
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