Can anyone help with yellow sample and very slow SDS page gel run?

I'm performing a western blot with samples that I need first to treat with a solution (yellow) to derivatize carbonyl groupS (the assay is called Oxyblot). My protein samples (bone protein...

11 December 2013 7,424 7 View

What is the right DAPI concentration to use in a flow cytometry experiment?

I need to check cell viability in a flow cytometry assay, and I always use PI. This time because I have different fluorochromes I can't use PI staining so I will use DAPI. But I have no idea about...

10 November 2013 6,029 6 View

Can an old Antibody lead to unspecific staining?

I'm using an Ab to detect a molecule on the membrane of the cells using flow cytometry. To confirm the specificity of the Ab I tested the Ab using 2 cell lines: one cell line has the molecule on...

04 May 2013 4,859 5 View

Do I need to do any compensation between APC and PI in flow cytometry?

I'm planning to do flow cytometry to check the purity of a B cell fraction sorted from bone marrow with magnetic beads. I will stain the B cell fraction with an APC-Ab anti CD19 and I will also...

31 December 2012 6,205 6 View

Cdna quantification

How can I measure cDNA concentration before the Real Time PCR? I need to know it because my amplification curves are very low and I guess that Rna retrotranscription step failed to work due to...

08 September 2011 6,979 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View