Marilina Piemontese

6 Questions 4 Answers 0 Followers

Questions related from Marilina Piemontese

Can anyone help with yellow sample and very slow SDS page gel run?

I'm performing a western blot with samples that I need first to treat with a solution (yellow) to derivatize carbonyl groupS (the assay is called Oxyblot). My protein samples (bone protein...

12 December 2013 7,514 7 View

What is the right DAPI concentration to use in a flow cytometry experiment?

I need to check cell viability in a flow cytometry assay, and I always use PI. This time because I have different fluorochromes I can't use PI staining so I will use DAPI. But I have no idea about...

11 November 2013 6,071 6 View

Can an old Antibody lead to unspecific staining?

I'm using an Ab to detect a molecule on the membrane of the cells using flow cytometry. To confirm the specificity of the Ab I tested the Ab using 2 cell lines: one cell line has the molecule on...

05 May 2013 4,896 5 View

Do I need to do any compensation between APC and PI in flow cytometry?

I'm planning to do flow cytometry to check the purity of a B cell fraction sorted from bone marrow with magnetic beads. I will stain the B cell fraction with an APC-Ab anti CD19 and I will also...

01 January 2013 6,246 6 View

ELISA assay detecting carbonylated protein in bone lysate sample

Does anybody know a good Elisa kit to detect carbonylated protein? My sample is a bone protein lysate. Any suggestion about the use oF ANTI-REDUCING AGENT? If my lysate buffer contains Triton...

05 May 2012 5,512 0 View

Cdna quantification

How can I measure cDNA concentration before the Real Time PCR? I need to know it because my amplification curves are very low and I guess that Rna retrotranscription step failed to work due to...

09 September 2011 7,012 0 View