We are trying to transfect primary retinal cell cultures (chicken) with a pCAG (Addgene) plasmid but we have very low transfection efficiency (
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
21 February 2021 5,601 3 View
I'm working on a study of how selection criteria of Tourism have changed considering Pandemic + Vaccination expectation, so propose a new prediction behavioral dynamic model for Riviera Maya (Mexico).
09 February 2021 314 7 View
Hello everybody! I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on: Rabino, I., and Mancinelli, A. L. 1986....
25 January 2021 3,686 2 View
Cronbach's alpha or McDonald's omega? When it comes to reliability, we find different methods for its estimation. Immediately, the name of Cronbach's Alpha coefficient arises, as it is the most...
21 January 2021 330 4 View
20 January 2021 7,043 2 View
We have a projet about TAVI
22 December 2020 7,729 1 View
I noticed that in many papers each author has a different human dummy to perform CFD simulation. However shouldn't this be standardize according to anthropomorphic data available? Right now, I...
12 December 2020 8,746 3 View
I been wondering if genes when stable transfected become regulated by the cell, or if the gene is constitutively express and translated and the protein previously made is degraded at a slower rate...
30 November 2020 8,095 2 View
We are two undergraduate students seeking for help!!! We are trying to simulate a laminar flux through a 3D pipe (25 cm long, 2 cm of diameter) consisting of a multiphase flux (air and water). We...
23 November 2020 3,918 3 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
02 March 2021 3,060 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View