I have a small well 384 plate from IDT with around ~10ul per well. The wells seem to evaporate? at the edges or certainly disappear over time?

Hi This is very odd. Whenever I come back to the plate and defrost it (At room temp for a couple of hours) wells that I know had previously been filled have become void. Some of them I have now...

05 June 2019 9,029 4 View

Can Magnessium Chloride be used to adhere DNA origami to TEM slides?

Hi Can magnessium chloride be used to adhere DNA origamis to TEM slides in the same way it does mica? Im very excited to get new images but im very unfamiliar with the process. Some papers dont...

31 December 2018 8,793 0 View

Im trying to purify gold nanoparticles from DNA in solution via pelleting and resuspending - at what RCF will ~20 base pair DNA begin to pellet?

Im functionalising gold nanoparticles with thiolated DNA, this is done using excess DNA in solution, it is critical that once the procedure is complete any remaining DNA is completely removed....

08 September 2018 7,887 5 View

Cleaning gold nanoparticles by centrifuge. Seems to end up as an aggregated mess?

Hi Im functionalising gold nanoparticles with thiolated oligos. Part of all (Or all that Im aware) protocols require spinning the final solution down at 13,300 rpm for 20 minute cycles to...

10 November 2017 2,542 4 View

Could litmus paper be used to adjust a small ~1ml solution from ~7 to ph 3 then back up to ~7?

I want to functionalise thiolated oligonucleotides with gold nanoparticles. To this I need to adjust the ph of the solution to 3, add Na+, mix, then bring the solution back to 7. I cant be sure...

08 September 2017 183 4 View

Can I use a PCR purification column to remove TCEP from a sample of reduced, thiolated, ssDNA sequences?

Can a PCR purification column be used in this manner? Thanks

08 September 2017 5,492 3 View

Will DNA origami aggregate in purified water or TE buffer?

Hi Hollow DNA spheres. Im not getting a good number to stick to mica for imaging and they seem to be aggregating when I try and get a size distribution of them in a zetasizer. Im trying to use a...

07 August 2017 3,247 2 View

As oppose to cutting out bands on an electrophoresis gel would a size exclusion column be a effective way to seperate DNA origami from excess staples?

04 May 2017 1,255 2 View

Matlab script to define N Z Mu and Epsilon from HFSS scatter parameters from a C resonator in the optical wavelength spectrum?

Hi I am having trouble accurately finding the refractive index of a structure I simulate in HFSS. I have been using chapter 3 of optical metamaterials (Wenshan Cai Vladimir Shalaev) to aid me in...

03 April 2017 633 2 View

Advice regarding SEM of DNA origami seeded with amine bound nanogold?

Hi I'm not having any success (Nothing visible on SEM) binding amine bound 1.4nm nanogold to DNA origami structures. I'm currently using a 100Kd protein concentration column, where origami and...

02 March 2017 1,004 4 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View

absorption= 1-mag(S(FP1:1,FP1:1)*S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)*S(FP2:1,FP1:1)) is it correct for absorption ?

hi everyone I am simulating a unit cell structure in HFSS using Flouquet port having two modes for absorption I have used this formula:- absorption=...

17 February 2021 9,178 2 View

Is there a way to use waveguide ports for circular antenna design in CST?

Hi, whenever i use circular array i get this error "Waveguide ports must be aligned with Cartesian coordinate planes for the transient solver" Does anyone use circular array with waveguide ports?...

09 February 2021 3,609 8 View

How to model in HFSS or FEKO Free Space Material Characterization?

I am having trouble setting up this problem in HFSS or FEKO. In HFSS, I've tried both FEM and FEBI-IE solvers. With FEKO, I've tried it's MoM solver and default settings. I am would like model...

09 February 2021 375 2 View

Using liquids as superstrate in Fabry-Perot Cavity Antenna?

Hi, A Fabry Perot Cavity allows to increase the directivity of a low gain antenna by putting in the top of cavity a superstrate with high permittivity such as a ceramic (er=10.2). However,...

09 February 2021 6,799 8 View

What is best solution for optical metamaterial design calculation: HFSS, COMSOL, CST?

Dear all, Now I'm selecting FEM for design of metamaterials on NIR~VIS frequencies, and keeping 3 software: ANSYS HFSS, COMSOL with wave optics module, and CST STUDIO SUITE. Could you give me...

09 February 2021 9,517 2 View