Hi:

I have tried lipo2000 and lipo3000, both works, and 3000 is better.

in 6 well plate, 4ug of plasmid, 4ul of lipo2000, or 4ug of plasmid, 8ul of P3000, 4ul of lipo3000.

Hi Mary,

For lipofectamine, you can check this and the manufacturer's protocol:

https://doi.org/10.1016/j.jconrel.2012.09.009