Mary Kilroy

5 Questions 3 Answers 0 Followers

Questions related from Mary Kilroy

Crystal Violet plates keep dying after DMSO treatment?

I have been trying to run crystal violet experiments for the past few months, but my cells keep dying immediately following the first treatment (even my DMSO control lane). I have tried increasing...

10 November 2022 6,820 0 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

26 February 2021 3,290 1 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

25 February 2021 1,974 4 View

Efficiently transfecting HeLa Cells?

I am currently looking to transfect HeLa cells using lentivirus and Lipofectamine 2000 and have been looking for cited protocols. Would anyone have any that they are willing to share?

01 November 2020 5,835 2 View

Lentiviral Production in COS-7 Cells

I am currently attempting to package lentivirus in COS-7 cells (I have ethical objections to 293 cells) and would welcome advice on how to proceed in my endeavor using Lipofectamine 2000 and a...

01 January 1970 1,621 3 View